Figure 1. Fibrosis and inflammatory cytokines are increased in skin of BLM-treated SKG mice. (A) Mice were given daily subcutaneous injections of BLM for 2 wk (BLM-treated BALB/c mice [n=5] and SKG mice [n=4]). (B) H&E staining and MT images of dermis thickness and collagen density in skin tissues of BLM-treated mice. Scale bars=100 μm. (C) Representative α-SMA and Col1-stained images of skin tissues of BLM-treated mice. Scale bars=100 μm. (D) Immunohistochemistry for IL-1β, IL-6, TNF-α, IL-4, IL-17, and IFN-α in skin tissues of BLM-treated mice. Scale bars=100 μm. Values are means ± SEMs from three independent experiments.

BLM, bleomycin.

^*p<0.05, ^**p<0.01, and ^***p<0.001.