Figure 3.

Pocket two of PLpro: structural analyses and relative activities.A–D, structural representations of key residues in Pocket 2 at the interface between the thumb, fingers, and palm domains. The residue labels are color-coded as described in Figure 2. The figure was produced using PyMOL Molecular Graphics System version 2.5.5 (Schrödinger LLC). E, bar plot of the relative activities of Pocket two mutants compared to WT. The relative activities were calculated from the slope of the linear line of the enzyme titration data in Fig. S2. The colors of the residues in A–D and the bars in E correspond to the domain colors established in Figure 1 (green = thumb, blue = fingers, and brown = palm). The data are presented as the mean ± SD, n = 3.