Figure 4.

Pocket three of PLpro: structural analyses and relative activities.A, bar plot of the relative activities of Pocket 3 mutants compared to WT. The relative activities were calculated from the slope of the linear line of the enzyme titration data in Fig. S3. B and C, Structural representations of key residues in Pocket 3, a cavity formed between the fingers and palm domains. The residue labels are color-coded as described in Figure 2. The figure was produced using PyMOL Molecular Graphics System version 2.5.5 (Schrödinger LLC). The colors of the bars in A and the residues in B and C correspond to the domain colors established in Figure 1 (blue = fingers and brown = palm). The data are presented as the mean ± SD, n = 3.