Figure 6.
Thermal inactivation kinetic measurements and half-life (t1/2) calculation. The residual enzymatic activity of each PLpro variant was measured at 15-min intervals while incubating the enzyme at 37 °C for up to 2 hours: (A) Pocket one mutants, (B–D) Pocket two mutants, and (E and F) Pocket three mutants. G, Bar plot of the half-life values of WT and mutant PLpro. The bar plot colors correspond to the domain colors established in Figure 1 (purple = Ubl, green = thumb, blue = fingers, and brown = palm). The data points represent the mean ± SD, n = 3.