Gpr84
−/−
mice show reduced weight gain under HFD feeding. (A) Plasma MCFA and LCFA contents determined by LC/MS in wild‐type mice (WT) and Gpr84
−/−
mice fed with NC or HFD for 5 weeks (NC‐fed group, n = 8; HFD‐fed group, n = 7; independent experiments). (B) Body weight changes in WT and Gpr84
−/−
mice fed with NC or HFD, and (C) Adipose tissue weight after 5 weeks (NC‐fed group, n = 5 from 8 litters; HFD‐fed group, n = 9 from 9 litters; independent experiments). Epi, epididymal adipose tissues; peri, perirenal adipose tissues; sub, subcutaneous adipose tissues; WAT, white adipose tissue; BAT, brown adipose tissue. (D) Tissue weight after 5 weeks (NC‐fed group, n = 5 from 8 litters; HFD‐fed group, n = 9 from 9 litters; independent experiments). (E) Food intake (NC‐fed group, n = 4 from 8 litters; HFD‐fed group, n = 9 from 9 litters; independent experiments). ***p < 0.001; **p < 0.01; *p < 0.05 (Tukey–Kramer test (A) Mann–Whitney U test (C, D, E) two‐way ANOVA with the Bonferroni (B). All data are presented as the mean ± SEM.