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. 2024 Oct 24;109:105409. doi: 10.1016/j.ebiom.2024.105409

Fig. 1.

Fig. 1

Development of quantitative YFV NS1 ELISA. (a) Sigmoidal standard curve of YFV NS1 capture ELISA performed with in-house-produced YFJ19 mAb and thirteen different concentrations of recombinant YFV NS1. The graph depicts mean absorbance values ± standard deviation (SD). (b) Comparison of standard curve of YFV NS1 diluted or not in normal human serum (1:10). The graph depicts mean absorbance values ± SD. (c) Specificity of mAb YFJ19 by direct ELISA performed with NS1 (5 μg/mL) from 12 different flaviviruses as follows: YFV; dengue virus serotypes 1 (DENV1), 2 (DENV2), 3 (DENV2), and 4 (DENV4); Saint Louis encephalitis virus (SLEV); West Nile virus (WNV); Zika virus (ZIKV); Wesselsbron virus (WBV); Usutu virus (USUV); Japanese encephalitis virus (JEV); Tick-borne encephalitis virus (TBEV). (d) Cross-reactivity of anti-flavivirus NS1 mAb (2B7) by direct ELISA performed with NS1 (5 μg/mL) from the 12 different flaviviruses as in c. (e) Western blot analysis showing specificity of mAb YFJ19 for YFV NS1 and cross-reactivity of mAb 2B7.