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. 2024 Oct 15;68(11):e00591-24. doi: 10.1128/aac.00591-24

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Gel electrophoresis depicts DNA fragments of varying sizes. Lane 1 is a molecular weight marker while lanes 2 to 7 depict DNA samples with different bands corresponding to fragment sizes. — Confirmation of the presence of linear plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) of optrA-positive enterococci. Lane 1, PFG MidRange Marker; lane 2, 2001206 (undigested); lane 3, 2001206 (digested); lane 4, 2001564 (undigested, ~114 kb linear plasmid present); lane 5, 2001564 (digested, ~114 kb linear plasmid present); lane 6, 2008300 (undigested, ~106 kb plasmid present); and lane 7, 2008300 (digested, ~106 kb plasmid present).