Figure 4.

LCN2 depletion promoted CLP-induced ferroptosis. Fe²⁺ concentration (A) and ROS levels (B) were assessed by ELISA in liver tissues of the control and CLP mice before and after LCN2 depletion. (C-E) PTGS2, SLC7A11, and GPX4 mRNA levels were assessed by qRT-PCR in liver tissues of the control and CLP mice before and after LCN2 depletion. (F and G) Western blot and quantitative analysis of PTGS2, SLC7A11, and GPX4 protein levels in liver tissues of the control and CLP mice before and after LCN2 depletion. Data were presented as mean ± SD (n = 5 per group). *p < 0.05, **p < 0.01, ***p < 0.001.