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. 2024 Nov 6;17:218. doi: 10.1186/s13048-024-01538-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — (a) ITGB8 splice graph positioning known variants and novel reads (denoted as dashed lines above and below exons, respectively); (b) Box and whisker plot representing isoform fractions of the alternative continuum of exons in the new variants; (c) pie chart showing the distribution of the number of dead and living patients expressing pITGB8-205; (d) Sanger sequencing-based validation of 133 bp ITGB8 (Exons 2, 4); (e) Novel 5’ pITGB8-205 sequences; (f) Predicted major U2-mediated spliceosome sites in pITGB8-205; (g.i) Overexpression of pITGB8-205 affects cell growth, cycling and metabolism. (a) Profiling of pITGB8-205 full length (2.4 kb) in 10 HGSC cell lines (X-axis indicates the HGSC cell lines, Y-Axis indicates the relative pITGB8-205 FL expression compared with the endogenous control; (g.ii) Screening of pITGB8-205 overexpressing (OVCAR3-OE, A4-OE) and knockdown (PEO14-KD) clones; (h) Bar graph representation of glucose consumption and lactate production (mg/dL; normalized with cell number) of OVCAR3-OE and control cells at 24, 48, 72, 96 and 120 h in culture; (i) Bar graph representing the cell cycle phases in OVCAR3-OE, A4-OE and PEO14-KD vs. control cells; (j) Histogram representing differential label quenching (cell cycling) dynamics of i-OVCAR3-OE, ii-A4-OE and iii-PEO14-KD vs. control cells (Solid lines- OVCAR3-OE/A4-OE/PEO14-KD, Dotted lines - respective controls at 0 (Green) and 96 h (black); (k) Representative doubling times of OVCAR3-OE, A4-OE and PEO14