Fig. 10.

Effects of VCE-004.8 on B55α and Sirt1 expression in vivo. A Representative double immunofluorescence confocal staining of B55α (red)/CD31 (green) (left) (scale bar equivalent to 50 µm) and immunofluorescence staining of Sirt1(green) (right) (scale bar equivalent to 20 µm) in gastrocnemius muscles 10 days after ischemia. B Quantifications of B55α (left) and Sirt1 (right). For B55α and Sirt1 quantification, the data do not pass the normality test performed, and the significance was determined non-parametric followed by a Kruskal–Wallis test. Values are mean ± SEM (n = 3–4). P values indicated in panels, significant as *p < 0.05 as **p < 0.01; ***p < 0.001. C Expression of B55α and Sirt1 mRNA in mice gastrocnemius at 10 days after femoral ligation. The data passed the normality test performed by the Shapiro–Wilk test. P value is calculated using 1-way ANOVA followed by Tukey post hoc multiple comparisons to compare between the groups (n = 3 animals per group). The results are shown as mean ± SEM. P value indicated in panel, significant as *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. D Representative immunofluorescence image of B55α (red) in cross-section of healthy and disease human arteries (left) (scale bar equivalent to 50 µm) and representative double immunofluorescence staining of Sirt1 (green) and CD31 (red) in cross-section of healthy and disease human arteries (right) (scale bar equivalent to 50 µm). Magnified region (white rectangle) showing details of Sirt1 expression (yellow arrow) (scale bar equivalent to 10 µm)