Fig. 2.

VCE-004.8 alleviates inflammation of vascular endothelial cells. A Representative image of VCAM, ZO-1, CLD1 and B55α expression in EA.hy926 cells stimulated for 24 h with proinflammatory cytokines and their quantifications. Scale bars (25 μm). The data passed the normality test performed by the Shapiro Wilk test. P value is calculated using 1-way ANOVA followed by Tukey post hoc multiple comparisons to compare between the groups (n = 3 independent experiments per group). B EA.hy926 cells preincubated with VCE-004.8 for 1 h and then treated with TNFα and IL6 for 24 h. ZO-1, CLD1 and B55α expression was analyzed by immunoblotting. Representative western blots of three independent analyses are shown. B55α and CLD1 protein expressions are normalized to actin. ZO-1 protein expression is normalized relative to vinculin. Results were expressed relative to control defined as 1. C VCAM-1 expression was analyzed by immunoblotting. Protein expression is normalized relative to actin and results were expressed relative to control defined as 1. The results are shown as mean ± SEM. P values indicated in panels, significant as **p < 0.01, ***p < 0.001 ****p < 0.0001