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. 2024 Oct 11;27(11):111156. doi: 10.1016/j.isci.2024.111156

Figure 6.

Figure 6

KCC2 is regulated by SNARE complex proteins

Immunoblot from neuro-2a cells transfected with KCC2 and scrambled shRNA (shScrambled), SNAP25 shRNA (shSNAP25-1), (A) VAMP1 shRNAs (shVAMP1-1, shVAMP1-2), or (B) VAMP2 shRNAs (shVAMP2-1, shVAMP2-2), or (C) STX1A shRNAs (shSTX1A-1, shSTX1A-2), or (D) STX1B shRNAs (shSTX1B-1, shSTX1B-2). The representative blot was probed with the antibodies indicated on the right. Summary graph (n = 3) showing total KCC2 abundance following (E) VAMP1 knockdown as shown in (A), (F) VAMP2 knockdown as shown in (B), (G) STX1A knockdown as shown in (C), (H) STX1B knockdown as shown in (D), normalized to shScrambled. Statistical significance was determined using ordinary one-way ANOVA with Tukey’s multiple comparisons test, with a single pooled variance. Asterisks denote significance from shScrambled. All graphs represent mean ± SEM. ns - not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.