Skip to main content
. 2024 Nov 5;6:64. doi: 10.1186/s42523-024-00350-3

Fig. 1.

Fig. 1

Ovary development in worker bees. (A) Microscope photographs demonstrating the four ovary developmental stages (0–3) of workers. Arrows point to the location of the ovaries on the photographs. Ovaries classified as stage 0 were indistinguishable from typical queen-right worker morphology and exhibited no visible thickening or oocyte development. Stage 1 ovaries demonstrated visible thickening but no constriction of oocyte development. Stage 2 ovaries were characterized by both visible thickening and constriction around at least one developed oocyte. Ovaries with multiple maturing eggs and across several ovarioles were categorized as 3. (B) Proportion of workers assigned to each ovary development stage from the four experimental groups. For statistical testing Stages 0–1 were considered to have undeveloped ovaries and Stages 2–3 were considered to have developed ovaries (*=P < 0.005, Chi-Squared Test)