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Alpha diversity in the worker gut microbiota compared across diets (A-C) and ovary developmental stages (D-F). Richness was measured based on the number of ASVs (A and D). Pielou’s evenness index was used to calculate microbiota evenness (B and E) and phylogenetic diversity was measured using Faith’s PD (C and F). Significance was tested using the Mann-Whitney test. *= P < 0.05, **= P < 0.005, ***= P < 0.0005
