Figure 2. Generation of APEX2-3XFlag knock-in at the dysf locus.

The APEX2-3XFlag-PBacDsRed fragment was inserted into the 5' end of the dysf coding region, creating dysf^JW . To generate an APEX2 knock-in tag, dysf^JW flies were crossed with BL8285 to excise the PBacDsRed fragment.