| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| Schneider's Drosophila medium | 500 mM | 445 mL |
| FBS | 10% | 50 mL |
| 100× Penicillin-Streptomycin | 1× | 5 mL |
| Total | n/a | 500 mL |
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