TABLE 1.
Probe pair name | Complementary to hairpin–spacer–complementary to RNA of interest |
---|---|
T1L-S3-P1a | GAGGAGGGCAGCAAACGG–AA–TGAGTGAGGAAGCCATAGTGACGAC |
T1L-S3-P1b | CTCTCTTGATCTTAGAGATCGCAGC–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P2a | GAGGAGGGCAGCAAACGG–AA–TCACGTTTAGCATACTGGAGCGGTC |
T1L-S3-P2b | TCCAGATTCCTTTGTCCAAGCAGAC–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P3a | GAGGAGGGCAGCAAACGG–AA–TAGCTGCAGATCAGCTCTCTCATGG |
T1L-S3-P3b | AACCCATCAGTGTGGTCGACCTTGA–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P4a | GAGGAGGGCAGCAAACGG–AA–CCATACCCATAGTGAACAATCTGGC |
T1L-S3-P4b | AAGGCTCAGTGGTAATGTGTAGTCC–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P5a | GAGGAGGGCAGCAAACGG–AA–AAGCTAACGTCATGCAGTCCAAATC |
T1L-S3-P5b | CACCATCAAGTGTAATCATGTAGGG–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P6a | GAGGAGGGCAGCAAACGG–AA–GCTAATCCCTTAAGTCCATCATCC |
T1L-S3-P6b | TCACATCCGTATGAGATATCCATGC–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P7a | GAGGAGGGCAGCAAACGG–AA–TTGATGCATCGTGAAGAATCCATGC |
T1L-S3-P7b | CTGCCGTTTCCTCGCAATATAACT–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P8a | GAGGAGGGCAGCAAACGG–AA–ATCAATGGTAGCAAATGAAGAAGCC |
T1L-S3-P8b | CATCACACAAAACCTGAAACACTGG–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P9a | GAGGAGGGCAGCAAACGG–AA–AGTCCAAAGTCTCATCGTTTCACGC |
T1L-S3-P9b | AACCACTGAATCGTCCAACGCCGAT–TA–GAAGAGTCTTCCTTTACG |
T1L-S3-P10a | GAGGAGGGCAGCAAACGG–AA–TCGACCTCGATCCAGCGAAATACTC |
T1L-S3-P10b | AGCATCAGTAGCATCAGCTGCTACC–TA–GAAGAGTCTTCCTTTACG |
Sequences for HCR probes specific for the reovirus S3 gene. Bold sequences indicate complementarity to the hairpin; italic sequences indicate complementarity to the RNA of interest.