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. 2024 Sep 25;9(10):e00629-24. doi: 10.1128/msphere.00629-24

TABLE 1.

Virus-specific HCR probesa

Probe pair name Complementary to hairpin–spacer–complementary to RNA of interest
T1L-S3-P1a GAGGAGGGCAGCAAACGGAATGAGTGAGGAAGCCATAGTGACGAC
T1L-S3-P1b CTCTCTTGATCTTAGAGATCGCAGCTAGAAGAGTCTTCCTTTACG
T1L-S3-P2a GAGGAGGGCAGCAAACGGAATCACGTTTAGCATACTGGAGCGGTC
T1L-S3-P2b TCCAGATTCCTTTGTCCAAGCAGACTAGAAGAGTCTTCCTTTACG
T1L-S3-P3a GAGGAGGGCAGCAAACGGAATAGCTGCAGATCAGCTCTCTCATGG
T1L-S3-P3b AACCCATCAGTGTGGTCGACCTTGATAGAAGAGTCTTCCTTTACG
T1L-S3-P4a GAGGAGGGCAGCAAACGGAACCATACCCATAGTGAACAATCTGGC
T1L-S3-P4b AAGGCTCAGTGGTAATGTGTAGTCCTAGAAGAGTCTTCCTTTACG
T1L-S3-P5a GAGGAGGGCAGCAAACGGAAAAGCTAACGTCATGCAGTCCAAATC
T1L-S3-P5b CACCATCAAGTGTAATCATGTAGGGTAGAAGAGTCTTCCTTTACG
T1L-S3-P6a GAGGAGGGCAGCAAACGGAAGCTAATCCCTTAAGTCCATCATCC
T1L-S3-P6b TCACATCCGTATGAGATATCCATGCTAGAAGAGTCTTCCTTTACG
T1L-S3-P7a GAGGAGGGCAGCAAACGGAATTGATGCATCGTGAAGAATCCATGC
T1L-S3-P7b CTGCCGTTTCCTCGCAATATAACTTAGAAGAGTCTTCCTTTACG
T1L-S3-P8a GAGGAGGGCAGCAAACGGAAATCAATGGTAGCAAATGAAGAAGCC
T1L-S3-P8b CATCACACAAAACCTGAAACACTGGTAGAAGAGTCTTCCTTTACG
T1L-S3-P9a GAGGAGGGCAGCAAACGGAAAGTCCAAAGTCTCATCGTTTCACGC
T1L-S3-P9b AACCACTGAATCGTCCAACGCCGATTAGAAGAGTCTTCCTTTACG
T1L-S3-P10a GAGGAGGGCAGCAAACGGAATCGACCTCGATCCAGCGAAATACTC
T1L-S3-P10b AGCATCAGTAGCATCAGCTGCTACCTAGAAGAGTCTTCCTTTACG
a

Sequences for HCR probes specific for the reovirus S3 gene. Bold sequences indicate complementarity to the hairpin; italic sequences indicate complementarity to the RNA of interest.