Figure 2. Removal of reward restructures ventral tegmental area (VTA) but not locus coeruleus (LC) input activity during spatial navigation.

(a) Experimental paradigm. (b, i) Population average position binned ${\displaystyle \mathrm{\Delta }F/F\pm \mathrm{s}.\mathrm{e}.\mathrm{m}.}$ of VTA regions of interest (ROIs) (n = 6 ROIs in 6 mice) in the rewarded (VTA rew, orange) and unrewarded conditions (VTA unrew, gray). Linear regression, F test, Rewarded, ${\displaystyle \mathrm{p}<1e-22}$, Unrewarded, ${\displaystyle \mathrm{p}<0.001}$. (ii) Same data as (b, i) binned by velocity. Linear regression, F test, Rewarded, ${\displaystyle \mathrm{p}<0.05}$, Unrewarded, ${\displaystyle \mathrm{p}<0.05}$. (iii) Same data as (b, i) aligned to motion onset. Linear regression, F test, Rewarded, ${\displaystyle \mathrm{p}<1e-4}$, Unrewarded, ${\displaystyle \mathrm{p}<0.05}$. (c, i) Population average position binned $\mathrm{\Delta }F/F\pm$ s.e.m. of LC ROIs (n = 26 ROIs in 7 sessions in 4 mice) in the rewarded (LC rew, blue) and unrewarded conditions (LC unrew, gray). Linear regression, F test, Rewarded, ${\displaystyle \mathrm{p}<0.01}$, Unrewarded, ${\displaystyle \mathrm{p}<0.01}$. (ii), Same data as (c, i) binned by velocity. Linear regression, F test, Rewarded, $P<1e-12$, Unrewarded, ${\displaystyle \mathrm{p}<1e-15}$. (iii) Same data as (c, i) aligned to motion onset. Linear regression, F test, Rewarded, ${\displaystyle \mathrm{p}<1e-21}$, Unrewarded, ${\displaystyle \mathrm{p}<1e-26}$. The slope of each unrewarded measure was compared to the familiar laps using a one-way ANCOVA with Tukey HSD post hoc test. *${\displaystyle \mathrm{p}<0.05}$, ***${\displaystyle \mathrm{p}<1e-4}$. This figure was created with BioRender.com.