Figure 3. Amino-terminal domain (ATD) splice affects the functional modulation of GluK1 kainate receptors by Neto proteins.

(A) Shows mean-weighted Tau (τ_Des) values calculated at 100 ms for GluK1-1a (red) and GluK1-2a (green), respectively, with full-length Neto1 (blue/light blue) or Neto2 (black/gray), in the presence of glutamate. Representative normalized traces are shown for 100 ms application of 10 mM glutamate for HEK293 cells co-expressing GluK1-1a or GluK1-2a with Neto1 and Neto2. (B) Shows Tau (τ_Recovery) values plotted for GluK1-1a and GluK-2a, respectively, with full-length Neto1 or Neto2. Relative amplitude graphs for each receptor in the absence or presence of Neto proteins are also depicted. (C) Demonstrates the glutamate dose-response curves for GluK1-1a with Neto proteins. (D) Indicates the ratio of peak amplitudes evoked in the presence of 1 mM kainate and 10 mM glutamate for GluK1-1a or GluK1-2a with or without Neto proteins. (E) The ratio of currents evoked by the application of 10 mM glutamate at +90 mV and –90 mV for the receptors in the absence or presence of Neto proteins is shown. (F) Shows representative IV plots for GluK1-1a and GluK1-2a for the receptor alone versus with Neto proteins, respectively. Panels (G) and (H) show data recorded from outside-out pulled patches. (G) Displays desensitization kinetics for GluK1-1a (red) and GluK1-2a (green) with or without Neto proteins, respectively. (H) Shows deactivation kinetics at 1 ms for GluK1-1a (red) and GluK1-2a (green) with or without Neto proteins. Error bars indicate mean ± SEM, N in each bar represents the number of cells used for analysis, and * indicates the significance at a 95% confidence interval.

Figure 3—figure supplement 1. Effect of Neto proteins on agonist-evoked responses of GluK1 receptors.

(A) Displays representative traces for GluK1-1a in the presence of Neto1 (blue) or Neto2 (black) with varying concentrations of glutamate (3 µM, 30 µM, 100 µM, and 1000 µM). Ligand application for receptors with Neto1 and Neto2 was 100 ms, and 1000 ms, respectively. (B) Representative 100ms traces for currents evoked in the presence of glutamate vs. kainate in the absence and presence of Neto proteins.