Table 2. Excised patch outside-out electrophysiology of GluK1-1a and GluK1-2a in the absence or presence of Neto1 (green) or Neto2 (peach).
Errors are reported as SEM. Statistical-significance is reported at 95% CI. p<0.05 (*), p<0.01 (**), p<0.001 (***), p<0.0001 (****) for comparisons between GluK1-1a and GluK1-2a receptors in presence of Neto proteins. Red p-values are statistical significance at 95% for comparison between GluK1-1a and GluK1-2a with either of the Neto proteins.
| Name of the construct | Deactivation | Desensitization | Total No. of cells tested | ||
|---|---|---|---|---|---|
| ƮDeact (ms) | Rise Time (ms) | ƮDes (ms) | Rise Time (ms) | ||
| GluK1-1a | Low peak amplitude | 0.68±0.19 (N=3) | Low peak amplitude | 0.48±0.1 (N=3) | 10 |
| +Neto1 | 2.83±0.2 (N=9) | 0.75±0.04 (N=9) (p=0.7615) | 4.83±0.46 (N=9) | 0.71±0.04 (N=9) | 9 |
| +Neto2 | 5.18±0.65 (N=3) | 1.5±0.24 (N=3) (p=0.0627) | 31.89±4.08 (N=4) | 1.87±0.06 (N=4) | 4 |
| GluK1-2a | 1.51±0.28 (N=4) | 0.58±0.06 (N=4) | 2.34±0.35 (N=5) | 0.72±0.07 (N=5) | 9 |
| +Neto1 | 2.14±0.37 (N=10) (p=0.2086) | 0.75±0.06 (N=10) (p=0.0731) | 2.84±0.69 (N=9) (p=0.5312 / *p=0.0310) | 0.69±0.07 (N=9) | 12 |
| +Neto2 | 10.74±1.48 (N=8) (***p=0.0004 / **p=0.0077) | 1.47±0.23 (N=8) (p=0062) | 20.91±2.11 (N=9) (***p=0.0003 / p=0.0665) | 1.42±0.25 (N=9) | 9 |