Figure 3.

Effect of astragaloside IV (AS-IV) on the phosphorylation of endothelial nitric oxide synthase (p-eNOS) in endothelial cells. Human umbilical vein endothelial cells (HUVECs) were incubated with AS-IV (30 µmol/L) for the indicated times. Cell lysates were immunoblotted with the antibodies against p-eNOS at Ser1177 or eNOS. (A) Representative western blot of p-eNOS in HUVECs. (B) Bar graph shows the relative optical density values for p-eNOS in HUVECs. Data were expressed as the ratio of p-eNOS to the corresponding eNOS. ⁣^∗p < 0.05 compared with the control group.