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. 2024 Jun 25;37(11):1970–1978. doi: 10.5713/ab.24.0210

Figure 1.

Figure 1

The PCR confirmation of Clostridium perferingens isolates and toxin genes detection using PCR. This figure demonstrates the results of PCR analyses used to confirm the presence of C. perfringens isolates and to detect specific toxin genes. (A) Shows successful amplification of the 16S rRNA gene in lanes 1–9, confirming the presence of C. perfringens. (B) Displays the amplification of the alpha toxin gene in lanes 1–9. (C and D) Show that there was no amplification for the epsilon and beta toxin genes, respectively, indicating these genes were not present in the tested isolates. M: DNA ladder,100 bp. PCR, polymerase chain reaction.