Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Nov 6;14:26884. doi: 10.1038/s41598-024-77164-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assay efficiency assessment. PCR efficiency represented by the best of fit regression line of the standard curve for the HGM targets. Cq – quantification cycles, the x-axis shows the concentration of standard target sequences in copies/µl. Blue dots in the curves represents standard target sequencies quantified at different concentrations. Assay threshold value for all targets was set at 0.01 except GBP6 whose threshold was set at 0.005 because it had low expression in clinical samples. Cycling A - the amplification cycle in the Yellow (HEX), Green (FIM), Crimson (ATTO700) and Orange (ROX) channel. Panel 1/2/3/4/5 are the multiplex reaction panels; Panel 1 to 3 each consisted of 4 HGM and panel 4 and 5 consisted of 2 HGM. R^2 value - coefficient of determination and the R value - Pearson correlation coefficient is the square root of R^2. M and B – represent the slope (M) and the intercept (B) of the standard curve. Efficiency – reaction efficiency by which the independent variable correlates with the dependent variable.