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. 2024 Nov 6;14:26884. doi: 10.1038/s41598-024-77164-5

Table 2.

Assay limit of detection and limit of quantification.

No. Gene Target LOD LOQ
Copies/µl Copies/µl Cq CV
1 ACTB 355 28.7 31.0 13.6%
2 ASUN 221 221 31.2 19.8%
3 ARG1 739 29.4 31.4 24.1%
4 BATF2 197 82.1 31.7 20.6%
5 C1QB 382 23.6 34.3 4.8%
6 CD64 178 64.6 28.8 4.4%
7 DHX29 354 29.4 33.6 18.8%
8 DUSP3 159 34.1 32.9 20.6%
9 GAS6 298 40.5 33.1 1.1%
10 GBP5 441 162 29.6 2.6%
11 GBP6 275 59.2 28.7 16.9%
12 KLF2 286 219 31.4 14.2%
13 NEMF 368 244 29.3 7.0%
14 PTPRC 328 23.2 31.0 11.3%
15 SEPT 282 282 31.0 1.8%
16 ZNF296 192 64.2 30.7 1.5%