FIGURE 1.

CB1 deletion affects functional/structural organization of the respiratory chain. (A) Representative immunoblots of CI–CV respiratory chain complex protein levels in liver mitochondria of CB1^+/+ and CB1^−/− mice. The protein level was normalized to that of VDAC. Histograms show the results of densiometric analysis of immunoblots. BN-PAGE-based analysis of individual respiratory complexes from dodecylmaltoside-solubilized crude mitochondria from the liver of CB1^+/+ and CB1^−/− mice. (B) Representative image of a Coomassie blue stained BN-PAGE gel. Bands characteristic of individual OXPHOS complexes are recognizable. Molecular weights of standard proteins and the relative position of the respiratory complexes (I–V) are indicated. (C) Representative images of histochemical staining of complex I (I), complex IV (IV), and complex II (II) in-gel activity. (D) Densitometric quantification of the blue bands corresponding to individual complexes (arbitrary units, a.u). (E–G) Densitometric quantification of bands corresponding to individual in-gel activity of complex I (E), IV (F), and II (G) (arbitrary units, a.u). (H) Activity of Cytochrome C Oxidase in liver mitochondria of CB1^+/+ and CB1^−/− mice. Protein extracts were prepared for each animal, and each individual was assessed separately. Protein load was 15 μg/lane. All values are represented as mean ± SEM; (n = 5/group). Student’s t-test was used for statistical analysis. p < 0.05 was considered significant. *p < 0.05 vs. CB1^+/+. Abbreviations: COX, Cytochrome C Oxidase.