Table 1. ¹H (600 MHz) and ¹³C (150 MHz) NMR Spectroscopic Data for Orfamide N (1) in d₃-MeOH.

unit	position	δC, type	δH, mult. [J (Hz)]	unit	position	δC, type	δH, mult. [J (Hz)]
l-Leu1	1	175.80, C		l-Leu7	1	172.29, C
	2	54.29, CH	4.00, m		2	53.69, CH	4.47, m
	3	40.69, CH2	1.67, m		3	41.86, CH2	1.80, m; 1.47, m
	4	25.87, CH	1.69, m		4	25.60, CH	1.74, m
	5	22.50, CH3	0.95, m		5	21.59, CH3	0.90, m
	6	23.23, CH3a	0.98, ma		6	23.82, CH3	0.96, m
	NH		8.66, brs		NH		7.94, m
d-Glu2	1	176.20, C		l-Leu8	1	174.95, C
	2	177.06, C			2	54.34, CH	4.29, m
	3	56.55, CH	4.01, m		3	41.46, CH2	1.72, m
	4	27.32, CH2	2.10, m; 2.01, m		4	25.92, CH	1.67, m
	5	34.57, CH2b	2.35, m		5	22.06, CH3	0.91, m
	NH		9.41, brs		6	23.37, CH3a	0.97, ma
					NH		7.90, m
d-allo-	1	173.11, C
Thr3	2	60.85, CH	4.30, m	d-Ser9	1	172.29, C
	3	70.66, CH	5.38, m		2	57.89, CH	4.28, m
	4	18.68, CH3	1.39, d (5.9)		3	62.62, CH2	3.89, m; 3.81, dd (11.3; 4.2)
	NH		8.20, d (7.2)		NH		8.04, brs
d-allo-	1	175.19, C		l-Val10	1	170.94, C
Ile4	2	62.62, CH	3.87, m		2	59.69, CH	4.33, m
	3	37.06, CH	2.05, m		3	31.46, CH	2.16, m
	4	27.01, CH2	1.46, m; 1.18, m		4	19.83, CH3	0.87, m
	5	11.77, CH3	0.91, m		5	18.95, CH3	0.82, d (6.7)
	6	16.40, CH3	0.98, m		NH		7.68, m
	NH		7.58, brs
				3′R–OH-hexadec-9-enoic acid	1′	175.02, C
d-Leu5	1	175.50, C			2′	44.52, CH2	2.43, m; 2.40, m
	2	54.97, CH	4.17, m		3′	69.89, CH	4.08, m
	3	40.89, CH2	1.79, m; 1.56, m		4′	38.55, CH2	1.50, m
	4	25.83, CH	1.79, m		5′	26.62, CH2	1.50, m
	5	21.14, CH3	0.87, m		6′	30.46, CH2a	1.28, ma
	6	23.69, CH3a	0.93, ma		7′	30.06, CH2a	1.36, ma
	NH		7.80, brs		8′	28.17, CH2	2.04, m
					9′	130.92, CH	5.35, m
d-Ser6	1	172.98, C			10′	130.79, CH	5.35, m
	2	58.33, CH	4.37, m		11′	28.17, CH2	2.04, m
	3	62.85, CH2	3.95, dd (11.6; 5.7);		12′	30.91, CH2a	1.28, ma
			3.89, m		13′	30.85, CH2a	1.28, ma
	NH		7.71, brs		14′	32.95, CH2aa	1.28, ma
					15′	23.73, CH2	1.31, m
					16′	14.46, CH3	0.89, m

^aThese four sets of chemical shifts are interchangeable.

^bShift acquired via analysis of HSQC data.