Table 2.
Positive and negative attributes of all mutations.
| Oxygen affinity p50 (torr) | Hill coefficient (h) | Autoxidation (min−1) | Heme release (min−1) | Ferric reduction (M−1 min−1) | Ferryl formation (s−1) | HPODE reactivity (s−1) | Liposome oxidation lag (min) | |
|---|---|---|---|---|---|---|---|---|
| F1 | 6.4 ± 0.1 | 2.53 ± 0.16 | 0.010 ± 0.003 | 0.054 ± 0.014 | 1.53 ± 0.16 | 12.76 × 10−3 ± 1.27 × 10−4 | 5.60 × 10−2 ± 7.55 × 10−4 | 123.38 ± 2.25 |
| F3 | 5.6 ± 0.2 | 1.95 ± 0.13 | 0.007 ± 0.0006 | 0.141 ± 0.025** | 1.10 ± 0.0032 | 19.38 × 10−3 ± 5.78 × 10−4 | 9.74 × 10−2 ± 8.19 × 10−4 | 513.40 ± 3.63*** |
| F5 | 8.0 ± 0.3 | 2.04 ± 0.17 | 0.023 ± 0.001* | 0.059 ± 0.004 | 1.49 ± 0.074 | 8.82 × 10−3 ± 9.08 × 10−5 | 4.48 × 10−2 ± 5.78 × 10−4 | 173.20 ± 0.52*** |
| F6 | 5.3 ± 0.3 | 1.81 ± 0.22 | 0.011 ± 0.001 | 0.080 ± 0.011 | 2.04 ± 0.076 | 9.99 × 10−3 ± 1.07 × 10−4 | 5.44 × 10−2 ± 6.59 × 10−4 | 267.27 ± 0.3*** |
| F7 | 19.8 ± 0.4 | 1.84 ± 0.11 | 0.016 ± 0.003 | 0.089 ± 0.008* | 5.93 ± 0.51 | 21.02 × 10−3 ± 1.36 × 10−3 | 8.59 × 10−2 ± 1.38 × 10−3 | 463.96 ± 2.55*** |
| F9 | 8.3 ± 0.3 | 1.66 ± 0.1 | 0.011 ± 0.002 | 0.074 ± 0.011 | 1.79 ± 0.064 | 14.75 × 10−3 ± 3.84 × 10−4 | 8.36 × 10−2 ± 1.19 × 10−3 | 263.15 ± 1.18*** |
| F10 | 8.4 ± 0.4 | 1.28 ± 0.09 | 0.008 ± 0.0004 | 0.097 ± 0.008** | 0.97 ± 0.12 | 10.02 × 10−3 ± 1.89 × 10−4 | 9.70 × 10−2 ± 7.18 × 10−4 | 229.00 ± 1.90*** |
For details of the assays, see the “Methods” section. For p50, ferric reduction, ferryl formation, and HPODE reactivity, the mean ± SEM was directly calculated from nonlinear fitting of the experimental data at different reactant concentrations. For autoxidation, heme release, and liposome oxidation lag times, the data are presented as the means ± SDs for studies performed in triplicate. In these latter cases, significance was compared to the F1 control (only) via an unpaired Student’s t test *p < 0.05; **p < 0.01; ***p < 0.001.