Table 3.
Comparison of the functional properties of F25, F41, and F45.
| F25 | F45 | F41 | ||
|---|---|---|---|---|
| p50 | p50 (torr) | 7.91 | 4.34 | ND |
| Hill coefficient (h) | 1.9 | 1.5 | ND | |
| Autoxidation (min−1) | 0.0022 ± 0.0005 | 0.0058 ± 0.0028*** | 0.0151 ± 0.0012*** | |
| Heme release (min−1) | 0.0300 ± 0.0092 | 0.0495 ± 0.0093* | 0.0487 ± 0.0075* | |
| Ferric reduction (M−1 min−1) | 2.10 ± 0.095 | 10.32 ± 0.045 | 9.14 ± 0.82 | |
| Liposome oxidation lag (min) | 269.1 ± 15.4 | 509.9 ± 11.7*** | 243.4 ± 8.9* | |
| NO dioxygenation (μM−1s−1) | 25.68 ± 1.11 | 2.69 ± 0.47*** | 2.89 ± 0.17*** | |
For details of the assays, see the methods section. ND, not determined. For ferric reduction, the mean ± SEM was directly calculated from nonlinear fitting of the experimental data to different concentrations of oxygen and ascorbate. For autoxidation, heme release, liposome oxidation lag time, and NO dioxygenation, the data are presented as the mean ± SD for studies performed in triplicate. In these latter cases, significance was compared to the F25 control (only) via an unpaired Student’s t test *p < 0.05; ***p < 0.001.