Fig. 2.
High glucose serum levels induced the progression of fibrosis in diabetic kidney tissue. (A, B) Representative images of fibronectin and α-SMA staining of kidney tissue in db/db 20-week-old mice (x200). Bar graph summarizing the % of the positive staining area. n = 10 each group. (C) Fibronectin and α-SMA protein levels in db/db 20-week-old mice were determined by Western blot. n = 4–6 each group. (D, E) Representative images of fibronectin and α-SMA staining of kidney tissue in STZ-induced diabetic models (x200). Bar graph summarizing the % of the positive staining area. n = 10 each group. (F) Fibronectin and α-SMA protein levels in STZ-induced diabetic models were determined by Western blot. n = 4–6 each group (The Kruskal–Wallis test, *p < 0.05, **p < 0.01, ***p < 0.001 vs. WT)