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. 2024 Nov 7;40:38. doi: 10.1186/s42826-024-00226-2

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Apoptosis and viability with NET or NET blocking in HK2 cells. (A) Annexin V-FITC and propidium iodide (PI) staining and (B) quantitative analyses for apoptosis after co-culture of neutrophils in HK2 cells. n = 4 each group. (C) MTT assay results according to NET blocking in HK2, CIHGM-1, and ciGEnC cells. n = 8 each group. (One-way ANOVA, ***p < 0.001 vs. con, ## p < 0.01, p < 0.001 vs. 20:1)