Fig. 2.

Overexpression of SLC38A5 induces cell proliferation, migration and invasion in osteosarcoma. (A) The qRT-PCR analysis of SLC38A5 in 143B and U2OS cells after infection of SLC38A5 overexpression lentivirus. (B-C) Western blot showed SLC38A5 protein levels in LV-SLC38A5 and LV-Control groups cells. (D-E) CCK-8 assay was conducted to evaluate the proliferation capacity of the LV-SLC38A5 and LV-Control groups cells. (F-G) Colony formation assay was performed to measure the colony formation ability of the two groups of cells. (H-I) Increased expression of SLC38A5 increased cell invasion ability based on transwell invasion assay, scale bar: 400 μm.(J-L) Representative images and quantitative analysis of wound healing assay showed the cell migration ability, scale bar: 200 μm. (M-O) Western blot analysis of EMT-related proteins including E-cadherin, N-cadherin and Vimentin. Results from three independent experiments are summarized in a histogram format. * P < 0.05, ** P < 0.01, *** P < 0.001