Fig. 7.

SLC38A5 inhibits ferroptosis in osteosarcoma cells through the glutamine-mediated PI3K/AKT/mTOR/SREBP1/SCD-1 axis. (A) The MDA level of the osteosarcoma cells in the four groups. (B) The ROS level of the osteosarcoma cells in the four groups. (C-D) The protein levels of SREBP1 and SCD-1 in osteosarcoma cells from the the LV-SLC38A5 and LV-Control group using western blot. (E-F) Western blot was used to evaluate SREBP1 and SCD-1 in the four groups. (G-J) Western blot was used to validate the knockdown efficiency of SREBP1 and SCD-1 via siRNA transfection. K-L) The MDA and ROS levels of each group, compared with the control group. Results from three independent experiments are summarized in a histogram format. * P < 0.05, ** P < 0.01, *** P < 0.001