Fig. 8.

SLC38A5 regulates tumor growth in vivo by mediating PI3K/AKT/mTOR signaling pathway and ferroptosis. (A) The growth curves of subcutaneous tumors in the shSLC38A5 and shNC groups. (B) Comparison of tumors obtained from nude mice of the two groups. (C) Tumor weight was compared between the two groups. (D-E Western blot and quantitative analyses of SLC38A5, p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR, E-cadherin, N-cadherin, Vimentin, Nrf2 and GPX4 in tumors. (F-G) IHC analysis of SLC38A5, p-mTOR, GPX4, N-cadherin for tissues of tumors, scale bar: 200 μm. Results from three independent experiments are summarized in a histogram format. * P < 0.05, ** P < 0.01, *** P < 0.001