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Antibodies α-SMA (eBioscience), calponin-h1 (Invitrogen), and SMMHC (eBioscience), respectively, were titrated against positive (HAoSMC) and negative (HUVEC and hiPSC) controls with A-C) Foxp3 staining kit with 2% FBS as the blocking buffer or D-F) Transcription Factor Set with 0.5% Human Fc Block.
