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Dilution for SMMHC antibody from A) Santa Cruz, B) Sigma, C) smoothelin-B (R&D), D) α-SMA (Sigma), and E) calponin-h1 (Sigma). All antibodies were titrated against positive and negative controls with Foxp3 staining kit. SMMHC (Santa Cruz) and smoothelin-B (R&D) were blocked with 2% FBS and 2% Human Fc for 30 minutes. SMMHC (Sigma), α-SMA (Sigma), and calponin-h1 (Sigma) were not blocked.
