Table 2. Details to be extracted from each included study.

Category	Extracted data	Details
Article journal	● Title ● Authors names ● Date of publication ● Journal ● Open access ● Definition of the task	Open access: Is the article published in an open access journal? yes or no Definition of the task: Report any definitions that authors have used to refer to prosociality or the task
Animals	● Ethical note ● Type of rodent ● Total # of subjects ● Total of males/females ● Age at arrival ● Strain ● Weight at arrival ● Food restriction ● Water restriction	Ethical note: Including the name of the animal care committee, the license number if applicable and the followed guidelines Type of rodent: Rats or mice Total of males/females: In case only one sex was tested, ‘NA’ is indicated for the non relevant sex Food and water restriction: Yes or no,—if yes, precision is given (e.g., 85% of free feeding body weight)
Housing conditions	● Number of animals per cage ● Relationship ● Dark-light cycle ● Enriched environment	Number of animals per cage: Modalities of animal housing–ex: Were the animals housed in pairs or more? If housed with more than one individual, specify whether the animal is housed with its experimental partner Relationship: Are the animals’ littermates or unrelated? Dark-light cycle: Indicate if the investigators specified the light/dark cycle (e.g., 12h, 16h, reversed) Enriched environment: Did the investigators use an enriched environment (yes or no). If yes, details if available are specified (description of the environment)
Methodology	● Operant paradigm ● Total of rats involved ● Groups ● Description of the task ● Type of task ● Computerized task ● Aversive or non-aversive ● Sensory paradigm (can rodents see/hear/smell each other) ● Duration of task (in minutes) ● Total of testing days ● Pretraining/habituation ● Control Group	Operant paradigm: Precise the selected operant paradigm (e.g., prosocial choice task, prisoner’s dilemma, freeing task, repeated donation game, etc.) Groups: Precise the group repartition Description of the task: Summary of the selected task Type of task: Cooperation, sharing, freeing, etc. Computerized task: Does the paradigm use an automated or computerized system? (e.g., operant box, video recording, ultrasonic vocalization, etc.) Aversive or non-aversive: Does the paradigm involve an aversive component (e.g., pain, fear, distress); if so, for one or both rats? (yes or no) Sensory elements: Do authors mention if rodents can see, hear or smell each other during the experimental task? (yes or no) Pretraining/Habituation: Is there a mention of a habituation or pretraining phase? (if yes, describe) Control group: Is a control group included? (if yes, describe)
Experimental interventions	● Drugs ● Surgery ● Diet ● Stress ● Other	Experimental interventions: Describe any use of drugs, diet, stress variables, surgery or other tests done during the course of the experiment (e.g., open field, elevated plus maze, etc.)
Results	● Performance index ● Behavioral analyses ● Sex differences ● Age differences ● Strain differences	Performance index: How did the study quantify the action? (e.g., lever presses, door openings, etc.) Behavioral analyses: Did the study use any metrics to analyze rodents’ behaviors? (e.g., video recording, ultrasonic vocalization) if yes, summarize the results. Sex differences: If the study used both sexes, summarize sex differences’ results if applicable Age differences: Summarize any results regarding age differences if applicable Strain differences: Summarize any results regarding strain differences if applicable