Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Rattus norvegicus, Sprague Dawley) | Sprague Dawley rat | Charles River | Strain Code 001; RRID: RGD_734476 | |
Recombinant DNA reagent |
PKA-Cα-mEGFP (plasmid) | Addgene | # 45524; RRID: Addgene_45524 | |
Recombinant DNA reagent |
PKA-RIα-mEGFP (plasmid) | Addgene | # 45525; RRID: Addgene_45525 | |
Recombinant DNA reagent |
PKA-RIβ-mEGFP (plasmid) | Addgene | # 45526; RRID: Addgene_45526 | |
Recombinant DNA reagent |
PKA-RIIα-mEGFP (plasmid) | Addgene | # 45527; RRID: Addgene_45527 | |
Recombinant DNA reagent |
PKA-RIα (plasmid) |
This paper | Figure 1—figure supplement 3 | |
Recombinant DNA reagent |
PKA-RIβ (plasmid) |
This paper | Figure 1 | |
Recombinant DNA reagent |
PKA-RIIα (plasmid) |
Addgene | #168492; RRID: Addgene_168492 | |
Recombinant DNA reagent |
PKA-RIIα-PKA-Cα-mEGFP (plasmid) |
This paper | Figure 2 | |
Recombinant DNA reagent |
shPKA against PKA-Cα with DsRed co-expression (plasmid) | This paper | The shRNA was developed in Tillo et al., 2017; Figure 3 | |
Recombinant DNA reagent |
shPKA against LacZ with DsRed co-expression (plasmid) | This paper | Figure 3 | |
Recombinant DNA reagent |
mCherry2 (plasmid) |
Addgene | #54517; RRID: Addgene_54517 |
|
Chemical compound, drug |
Norepinephrine | Tocris | 5169 | |
Chemical compound, drug |
Forskolin | LC Labs | F-9926 | |
Chemical compound, drug |
IBMX | Sigma-Aldrich | I7018 | |
Chemical compound, drug |
MNI-glutamate | Tocris | 1490 | |
Chemical compound, drug |
TTX | Tocris | 1069 | |
Chemical compound, drug |
2-Chloroadenosine | Sigma-Aldrich | C5134 | |
Chemical compound, drug |
GABAzine (SR 95531) | Tocris | 1262 | |
Software, algorithm | MATLAB | MathWorks | RRID: SCR_001622 | |
Software, algorithm | SI_View | Zhong, 2022 | https://github.com/HZhongLab/SI_View |