Video 1. Release of a chloroplast bud as visualized by chloroplast stroma–targeted GFP.

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The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma–targeted GFP (CT-GFP) was incubated in sugar-free solution in darkness for time-lapse imaging with a two-photon excitation microscope equipped with a confocal spinning-disk unit. Arrowhead indicates a chloroplast budding structure. Three-dimensional (3D) reconstructed images (8 µm in depth) acquired about every 3 s are displayed at 10 frames/s. Scale bar, 5 µm. This video was used to generate Figure 1A.