Video 16. Autophagosome development and chloroplast segmentation occur concomitantly.

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The second rosette leaf from a 23-day-old plant accumulating the chloroplast stroma–targeted DsRed (CT-DsRed) and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness for time-lapse imaging. Arrowhead indicates the portion of the chloroplast-associated isolation membrane. Images acquired every 1 s are displayed at 20 frames/s. Scale bar, 5 µm. Green, GFP-ATG8a; magenta, CT-DsRed. Only the video of the merged channels is shown. This video was used to generate Figure 6B.