Video 18. Autophagy-related chloroplast segmentation occurs in sequence.

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The second rosette leaf from a 21-day-old plant accumulating the chloroplast stroma marker RBCS-tagRFP and the isolation membrane marker GFP-ATG8a was incubated in sugar-free solution in darkness for time-lapse imaging. White, yellow, and blue arrowheads indicate the isolation membrane−associated sites in different chloroplasts. Images acquired every 2 s are displayed at 10 frames/s. Scale bar, 5 µm. Green, GFP-ATG8a; magenta, RBCS-tagRFP. Only the video of the merged channels is shown. This video was used to generate Figure 6—figure supplement 2.