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. 2024 Oct 28;13(10):5484–5496. doi: 10.21037/tcr-24-248

Figure 2.

Figure 2

ISL1 promotes gastric cancer development in vitro. ISL1 was knocked down or overexpressed in the AGS or HGC-27 cell lines, and the cells were analyzed to measure cell proliferation (A-D, A and B are AGS cells, C and D are HGC-27 cells); cell migration: the cells were stained using crystal violet dye and then observed and photographed under a 10× lens (E-H, E and F are AGS cells, G and H are HGC-27 cells); colony formation: direct observation and photographing of cells after staining with crystal violet dye (I-L, I and J are AGS cells, K and L are HGC-27 cells) (biological repeat 3 times). ***, P<0.001. OD, optical density; Ad vector, empty vector control of exogenous overexpression of ISL1; Ad ISL1, exogenous overexpression of ISL1; sh control, empty vector control of knockdown of ISL1 expression; sh ISL1, knockdown of ISL1 expression; SOX2, SRY-box transcription factor 2; CD44, CD44 molecule; OCT4, POU class 5 homeobox 1; ISL1, insulin gene enhancer binding protein-1.