Figure 4.

ISL1 promotes the tumorigenicity and metastatic capacity of GC cells in vitro. ISL1 was overexpressed in AGS cells. (A) These cells were diluted and subcutaneously injected into severely immunodeficient mice. Tumors were examined over a 28-day period (n=3 for each group, biological repeat 3 times). The tumor occurrence time (B), volume (C,D) and tumor weight (E) were monitored in the indicated groups and at the indicated time points. (F) ISL1 overexpressing and control cells (GFP labeled) were injected into the tail vein of mice, the number of metastatic nodules in the lungs of mice was observed (using the stereomicroscope) after 28 days, and the number of metastatic nodules in the lungs of mice was counted (biological repeat 3 times). Ad vector, empty vector control of exogenous overexpression of ISL1; Ad ISL1, exogenous overexpression of ISL1; ISL1, insulin gene enhancer binding protein-1; GC, gastric cancer; GFP, green fluorescent protein.