Table 1.

Accuracy and precision.

Sample/peptides level	Theoretical concentration (µg/ml)	Measured concentration (µg/ml)	Recovery (%) (Accuracy)	Intra-assay RSD (%) (Repeatability)	Average% RSD
Amino acids solution samples
Low	30	32.68	109	0.74	0.88
Medium	60	56.36	94	0.77
High	105	106.36	101	1.14

RSD—Relative standard deviation. All samples were run in quadruplicate.