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. 2024 Oct 25;12:1447630. doi: 10.3389/fcell.2024.1447630

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Copyright © 2024 Sanovec, Frolikova, Kraus, Vondrakova, Qasemi, Spevakova, Simonik, Moritz, Caswell, Liska, Ded, Cerny, Avidor-Reiss, Hammoud, Schorle, Postlerova, Steger and Komrskova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acrosome formation is aberrant in Prm2^−/− seminiferous tubules. 3D visualization of surface-rendered sperm acrosomes (green, PNA-AF488 stained) and nuclei (blue, DAPI stained) in the seminiferous tubule. Surface rendering was postprocessed by Imaris software. The upper panel shows the overall look of half of the seminiferous tubule with surface-rendered acrosomes only. The lower panel shows details of spermatids nuclei and acrosomes in the seminiferous tubule with surface-rendered acrosomes and nuclei.