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. 2024 Oct 25;12:1447630. doi: 10.3389/fcell.2024.1447630

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Copyright © 2024 Sanovec, Frolikova, Kraus, Vondrakova, Qasemi, Spevakova, Simonik, Moritz, Caswell, Liska, Ded, Cerny, Avidor-Reiss, Hammoud, Schorle, Postlerova, Steger and Komrskova.

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Cytoskeletal protein abundance and gene expression in Prm2^−/− sperm and testes is modified for β-Actin, and Septin12 subcellular localization is altered. (A, D) Western blots of specified cytoskeletal proteins in testes (A) or sperm (D) of WT and Prm2^−/− mice. Blue arrows indicate the molecular size of the antibody-detected bands. (B, E) A relative protein amount quantification. Band intensities were normalized to the CBB-stained membrane. n = 5, Mann-Whitney test, **p = 0.0079. (C) qPCR of total testes lysate showing no difference in relative gene expression of all individual Septin 12 isoforms and total Septin 12. n = 8, Mann-Whitney test. (F) Detection of Septin 12 in subcellular fractions of WT and Prm2^−/− sperm. CF - Cytoplasmic fraction; MF - Membrane fraction; NF - Nuclear fraction; ChNF - Chromatin-bound nuclear fraction; T/MtF -Tubulin/mitochondria protein fraction. Blue arrows indicate the molecular size of the antibody-detected bands.