Table 1. PCR reaction for amplification of sgRNA DNA template.
| Reagent | Volume |
|---|---|
| 2× Taq Master Mix (Dye Plus) | 25 μL |
| sgRNA primer (10 μm) | 2 μL |
| Universal primer (10 μm) | 2 μL |
| Deionized H2O | 21 μL |
| Total volume | 50 μL |
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