Table 2. PCR program for amplification of sgRNA DNA template.
| Temperature | Time | Cycle number |
|---|---|---|
| 94 °C | 3 min | 1 cycle |
| 94 °C | 15 s | 45 cycles |
| 50 °C | 15 s | |
| 72 °C | 30 s | |
| 72 °C | 5 min | 1 cycle |
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