Table 6. Combination of U6 vectors and pGGDestISceIEG-XsgRNA for different number of sgRNAs.
sgRNA number | U6 vectors | pGGDestISceIEG-XsgRNA |
---|---|---|
1 | pU6a:sgRNA#1 | pGGDestISceIEG-1sgRNA |
2 | pU6a:sgRNA#1, pU6a:sgRNA#2 | pGGDestISceIEG-2sgRNA |
3 | pU6a:sgRNA#1, pU6a:sgRNA#2, pU6b:sgRNA#3, | pGGDestISceIEG-3sgRNA |
4 | pU6a:sgRNA#1, pU6a:sgRNA#2, pU6b:sgRNA#3, pU6c:sgRNA#4 | pGGDestISceIEG-4sgRNA |