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. 2024 Nov 5;14(21):e5092. doi: 10.21769/BioProtoc.5092

Table 6. Combination of U6 vectors and pGGDestISceIEG-XsgRNA for different number of sgRNAs.

sgRNA number U6 vectors pGGDestISceIEG-XsgRNA
1 pU6a:sgRNA#1 pGGDestISceIEG-1sgRNA
2 pU6a:sgRNA#1, pU6a:sgRNA#2 pGGDestISceIEG-2sgRNA
3 pU6a:sgRNA#1, pU6a:sgRNA#2, pU6b:sgRNA#3, pGGDestISceIEG-3sgRNA
4 pU6a:sgRNA#1, pU6a:sgRNA#2, pU6b:sgRNA#3, pU6c:sgRNA#4 pGGDestISceIEG-4sgRNA