Table 8. Program for ligating sgRNA oligos to U6 vectors.
| Temperature | Time | Cycle number |
|---|---|---|
| 37 °C | 20 min | 6 cycles |
| 16 °C | 15 min | |
| 37 °C | 10 min | 1 cycle |
| 55 °C | 15 min | 1 cycle |
| 80 °C | 15 min | 1 cycle |
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