Table 9. PCR reaction for identification of positive colony.
| Reagent | Volume |
|---|---|
| 2× Taq Master Mix (Dye Plus) | 5 μL |
| Colony liquid | 2 μL |
| M13 forward (10 μm) | 0.4 μL |
| Reverse primer (10 μm) | 0.4 μL |
| Deionized H2O | 2.2 μL |
| Total volume | 10 μL |
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